OBSERVATIONS ON THE SUBCELLULAR LOCALIZATION OF OXIDATIVE ENZYMES WITH NITRO BLUE TETRAZOLIUM

Mitochondrial Localization of Oxidative Enzymes: Staining Results with Two Tetrazolium Salts

A comparison is made of the staining results obtained with Nitro-BT and MTT-Co(++) as acceptors when DPNH is the substrate in frozen sections of cold formol-calcium-fixed rat kidney (normal and following ligation of the blood vessels) and human liver containing lipofuscin granules. The kidney results are evaluated in terms of mitochondrial morphology seen after classical mitochondrial stains an...

Nitro blue tetrazolium test in children with kwashiorkor with a comment on the use of latex particles in the test.

The nitro blue tetrazolium test was applied on neutrophils from children with kwashiorkor and from control children. The percentage of formazan cells formed in the kwashiorkor patients was significantly less than in the control group in spite of the presence of associated infections in the former. A direct correlation exists between the percentage of formazan cell formation and total serum prot...

Actions of adenosine on nitro blue tetrazolium deposition and surface pH during intestinal reperfusion injury.

Mesenteric arteries supplying an intestinal segment were occluded for 5 minutes and then released. During reperfusion, two series of measurements were made with various substances topically applied to the extraluminal surface. In the first series, reduced nitro blue tetrazolium (NBT) was extracted from tissue and measured spectrophotometrically, as an index of oxidative damage. In the second se...

Technique for the performance of the nitro-blue tetrazolium (NBT) test.

Failure of nitro blue tetrazolium reduction in the phagocytic vacuoles of leukocytes in chronic granulomatous disease.

The leukocytes of patients with chronic granulomatous disease (CGD) may be identified by their failure to reduce Nitro Blue Tetrazolium (NBT) during phagocytosis. This reaction, normally detected in the phagocytic vacuole, is absent or delayed in CGD monocytes and eosinophils as well as in neutrophils, even though sonicates of normal and CGD leukocytes contain equal activities of a cyanide inse...